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BUSM Study discovers novel therapeutic targets for Huntington’s disease

  • - Dementia News
  • Feb 28, 2014
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Tags: | fatal neurological disorder | huntington's disease |

A study led by researchers at Boston University School of Medicine (BUSM) provides novel insight into the impact that genes may have on Huntington’s disease (HD). The study, published online in PLOS Genetics, identified specific small segments of RNA (called micro RNA or miRNA) encoded in DNA in the human genome that are highly expressed in HD. Micro RNAs are important because they regulate the expression of genes. The researchers showed that these miRNAs are present in higher quantities in patients with HD and may act as a mitigating factor in the neurologic decline associated with the disease, making them a possible therapeutic target.

HD is an inherited and fatal neurological disorder that is usually diagnosed when a person is between 30 and 50 years old. Huntingtin, the single gene mutation responsible for the disease, was identified in 1993.

The investigators examined 21 autopsy brain samples: 12 with HD and nine without. Genetic sequencing analyses were performed on these brain tissues, including quantifying the amount of all the microRNAs present in the brain and their corresponding gene or messenger RNA (mRNA) counterparts. This information was combined with a genetic study to characterize variations in the HD gene. The researchers also gathered the clinical neurological information on the patients’ age when HD symptoms presented and how long the patient survived with the disease.

Based on this analysis, the investigators discovered increased amounts of four miRNAs were expressed in the brains of HD patients and that the amount of miRNA was highly correlated with disease status. An increased amount of miRNA in brain cells was correlated with a younger age at disease onset and an earlier age at death of the patients.

“The genes which these miRNAs regulate also had increased levels, indicating that these gene expression, indicating that these gene products were likely targeted for storage and for possible future use within the brain cell, rather than for destruction. When we experimentally increased the expression of the microRNAs in model nerve cells designed to replicate the conditions of HD, the cells lived longer, indicating that these miRNAs may promote cell survival,” explained lead author Richard Myers, PhD, professor of neurology at BUSM. The authors conclude that these genes may represent new therapeutic targets for HD.

Huntington disease is a disorder in which nerve cells in certain parts of the brain waste away, or degenerate. The disease is passed down through families.
Causes

Huntington disease is caused by a genetic defect on chromosome 4. The defect causes a part of DNA, called a CAG repeat, to occur many more times than it is supposed to. Normally, this section of DNA is repeated 10 to 28 times. But in persons with Huntington disease, it is repeated 36 to 120 times.

As the gene is passed down through families, the number of repeats tend to get larger. The larger the number of repeats, the higher your chance of developing symptoms at an earlier age. Therefore, as the disease is passed along in families, symptoms develop at younger and younger ages.

There are two forms of Huntington disease.

  Adult-onset Huntington disease is the most common. Persons with this form usually develop symptoms in their mid 30s and 40s.
  Early-onset Huntington disease affects a small number of cases and begins in childhood or the teens.

If one of your parents has Huntington disease, you have a 50% chance of getting the gene. If you get the gene from both your parents, you will develop the disease at some point in your life. You will also pass it on to your children. If you do not get the gene from your parents, you cannot pass the gene on to your children.

According to the researchers, these miRNA sequences were found to be present in much higher levels in patients with HD (some were undetectable in the brain cells of normal patients), which would also make them excellent targets as biomarkers for HD expression. “If this miRNA were also found outside of brain tissue, for example in the blood, it could be used as an inexpensive, non-invasive assessment of the severity of the disease and perhaps for evaluating the effectiveness of treatments in clinical trials for HD. If the amount of miRNA were quantified in an HD patient, the amount could provide insight into the likely age of disease onset or life expectancy of the patient which current genetic testing in HD does not provide,” added Myers.

Huntington’s Disease Signs and Symptoms

Huntington’s disease produces three types of symptoms: movement, cognitive, and psychiatric. The sequence in which symptoms develop varies from person to person.

Movement and Huntington’s Disease

Uncontrolled movement, or tics, may develop in the fingers, feet, face, or trunk. This is the beginning stage of chorea—involuntary, rapid, ceaseless movement. Chorea can become more intense when the person is anxious or disturbed. Over time other symptoms, such as the following, emerge:

  Clumsiness
  Jaw clenching (bruxism)
  Loss of coordination and balance
  Slurred speech
  Swallowing and/or eating difficulty
  Uncontrolled continual muscular contractions (dystonia)
  Walking difficulty, stumbling, falling

Cognitive Function and Huntington’s Disease

Over time judgment, memory, and other cognitive functions begin to deteriorate into dementia. As Huntington’s disease progresses, the ability to concentrate becomes more difficult. The person may have difficulty driving, keeping track of things, making decisions, answering questions, and may lose the ability to recognize familiar objects.

Psychiatric Symptoms of Huntington’s Disease

Early psychiatric symptoms of Huntington’s disease are subtle, varied, and easily overlooked or misinterpreted. Depression is the most common psychiatric symptom of Huntington’s and often develops early in the course of the disease. Signs of depression include:

  Hostility/irritability
  Inability to take pleasure in life (anhedonia)
  Lack of energy

Some people develop manic-depression, or bipolar disorder, during the course of the disease.

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This genetic study is the product of a collaboration between the lead investigators at BUSM and their colleagues at Massachusetts General Hospital, Mount Sinai School of Medicine and the University of Massachusetts Medical School.

This research was supported by the National Institute of Neurological Disorders and Stroke under award number 1R01-NS073947 and the Jerry McDonald Huntington’s Disease Research Fund.

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Gina DiGravio
.(JavaScript must be enabled to view this email address)
617-638-8480
Boston University Medical Center

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